Journal: Journal of Cellular and Molecular Medicine

Article Title: Gastrodin induces lysosomal biogenesis and autophagy to prevent the formation of foam cells via AMPK‐FoxO1‐TFEB signalling axis

doi: 10.1111/jcmm.16600

Figure Lengend Snippet: Gastrodin rescues lysosomal dysfunction and autophagy deficiency in foam cells. A and B, Gastrodin‐induced lysosomal biogenesis in the foam cells. Protein level of LAMP1 was determined by Western blotting A, and immunofluorescence B. C and D, Gastrodin rescued the lysosomal dysfunction of foam cells. Gastrodin enhanced LysoSensor staining C, and increased the protein level of CTSD D. E‐G, Gastrodin rescued the impaired autophagy of foam cells. Expression levels of autophagic proteins (LC3I/II, p62 and Beclin1) were analysed by Western blotting in macrophages treated with gastrodin and ox‐LDL in the absence E, and presence F, of Baf A1 (25nM). G, Gastrodin increased the number of autolysosomes in the foam cells as shown by the elevated red puncta of mRFP‐GFP‐LC3. * P < .05; ** P < .01. Results are means ± SD of three independent experiments. The value represents fold of vehicle

Article Snippet: LAMP1 (1:1000; catalog no. A16894), ABCA1 (1:1000; catalog no. A16337), IL‐18 (1:1000; catalog no. A16737), Beclin 1 (1:1000; catalog no. A11761), CD36 (1:1000; catalog no. A5792), TNF‐α (1:1000; catalog no. A11534) and CTSD (1:1000; catalog no. A13292) were purchased from ABclonal Technology (Wuhan, China).

Techniques: Western Blot, Immunofluorescence, Staining, Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Gastrodin induces lysosomal biogenesis and autophagy to prevent the formation of foam cells via AMPK‐FoxO1‐TFEB signalling axis

doi: 10.1111/jcmm.16600

Figure Lengend Snippet: TFEB is involved in gastrodin‐induced lysosome biogenesis and autophagy. A and B, The expression of TFEB was determined after transfecting by control shRNA and shRNA against TFEB. A, The mRNA level of TFEB. B, The protein level of TFEB. C‐E, Inhibition of TFEB expression abolished the effect of gastrodin on lysosomal biogenesis and lysosomal function in the foam cells. C, The protein levels of LAMP1 and CTSD. D, Immunofluorescence analysis of LAMP1. E, Measurement of lysosomal pH. F, Inhibiting TFEB expression reduced the protein levels of LC3II and Beclin 1 and increased the protein level of p62. * P < .05. Results are presented as mean ± SD of three independent experiments. The value represents fold of vehicle. shCont., Control shRNA

Article Snippet: LAMP1 (1:1000; catalog no. A16894), ABCA1 (1:1000; catalog no. A16337), IL‐18 (1:1000; catalog no. A16737), Beclin 1 (1:1000; catalog no. A11761), CD36 (1:1000; catalog no. A5792), TNF‐α (1:1000; catalog no. A11534) and CTSD (1:1000; catalog no. A13292) were purchased from ABclonal Technology (Wuhan, China).

Techniques: Expressing, Control, shRNA, Inhibition, Immunofluorescence